This research explores antimicrobial activity of rose petal tinctures. Since bacteria are mutating and becoming more resistant to common antimicrobial drugs, natural remedies could be a viable option for identifying new compounds to be used to fight off infectious bacteria. Similar research has been conducted in this area but with only select rose varieties such as R. damascene and R. rugosa. The research being conducted covers a wider range of rose varieties. Rose petal tinctures were prepared in 50% lab grade ethanol. Tinctures are being tested with 96 well plate growth assays to obtain growth curves. The bacteria being used in this analysis include two that are gram negative: E. coli and B. subtilis, and two gram positive: M. luteus, and P. aeruginosa. The results show promising outcomes as some samples already have shown bacterial inhibition, which indicates this research could lead to a new method of fighting bacteria.